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Journal of Biomedical Optics

D38-cholesterol as a Raman active probe for imaging intracellular cholesterol storage
Author(s): Alba Alfonso García; Simon G. Pfisterer; Howard Riezman; Elina Ikonen; Eric O. Potma
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Paper Abstract

We generated a highly deuterated cholesterol analog (D38-cholesterol) and demonstrated its use for selective vibrational imaging of cholesterol storage in mammalian cells. D38-cholesterol produces detectable signals in stimulated Raman scattering (SRS) imaging, is rapidly taken up by cells, and is efficiently metabolized by acyl-CoA cholesterol acyltransferase to form cholesteryl esters. Using hyperspectral SRS imaging of D38-cholesterol, we visualized cholesterol storage in lipid droplets. We found that some lipid droplets accumulated preferentially unesterified D38-cholesterol, whereas others stored D38-cholesteryl esters. In steroidogenic cells, D38-cholesteryl esters and triacylglycerols were partitioned into distinct sets of lipid droplets. Thus, hyperspectral SRS imaging of D38-cholesterol demonstrates a heterogeneous incorporation of neutral lipid species, i.e., free cholesterol, cholesteryl esters, and triacylglycerols, between individual lipid droplets in a cell.

Paper Details

Date Published: 17 December 2015
PDF: 8 pages
J. Biomed. Opt. 21(6) 061003 doi: 10.1117/1.JBO.21.6.061003
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Alba Alfonso García, Univ. of California, Irvine (United States)
Simon G. Pfisterer, Univ. of Helsinki (Finland)
Howard Riezman, Univ. de Genève (Switzerland)
Elina Ikonen, Univ. of Helsinki (Finland)
Eric O. Potma, Univ. of California, Irvine (United States)


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