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Journal of Biomedical Optics

Cell optoporation with a sub-15 fs and a 250-fs laser
Author(s): Hans Georg Breunig; Ana Batista; Aisada Uchugonova; Karsten König
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Paper Abstract

We employed two commercially available femtosecond lasers, a Ti:sapphire and a ytterbium-based oscillator, to directly compare from a user’s practical point-of-view in one common experimental setup the efficiencies of transient laser-induced cell membrane permeabilization, i.e., of so-called optoporation. The experimental setup consisted of a modified multiphoton laser-scanning microscope employing high-NA focusing optics. An automatic cell irradiation procedure was realized with custom-made software that identified cell positions and controlled relevant hardware components. The Ti:sapphire and ytterbium-based oscillators generated broadband sub-15-fs pulses around 800 nm and 250-fs pulses at 1044 nm, respectively. A higher optoporation rate and posttreatment viability were observed for the shorter fs pulses, confirming the importance of multiphoton effects for efficient optoporation.

Paper Details

Date Published: 1 June 2016
PDF: 4 pages
J. Biomed. Opt. 21(6) 060501 doi: 10.1117/1.JBO.21.6.060501
Published in: Journal of Biomedical Optics Volume 21, Issue 6
Show Author Affiliations
Hans Georg Breunig, JenLab GmbH (Germany)
Ana Batista, JenLab GmbH (Germany)
Univ. des Saarlandes (Germany)
Aisada Uchugonova, JenLab GmbH (Germany)
Univ. des Saarlandes (Germany)
Karsten König, JenLab GmbH (Germany)
Univ. des Saarlandes (Germany)

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