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Journal of Biomedical Optics • Open Access

Photoacoustic molecular imaging for in vivo liver iron quantitation
Author(s): Federica Maccarinelli; Fernando Carmona; Maria Regoni; Paolo Arosio

Paper Abstract

A recent study showed that ferritin is a suitable endogenous contrast agent for photoacoustic molecular imaging in cultured mammalian cells. We have therefore tested whether this imaging technique can be used for in vivo quantification of iron in mouse livers. To verify this hypothesis, we used multispectral optoacoustic tomography (MSOT) to image albino CD1 mice before and after experimental iron loading. Postmortem assays showed that the iron treatment caused a 15-fold increase in liver iron and a 40-fold increase in liver ferritin levels, while in vivo longitudinal analysis using MSOT revealed just a 1.6-fold increase in the ferritin/iron photoacoustic signal in the same animals. We conclude that MSOT can monitor changes in ferritin/iron levels in vivo, but its sensitivity is much lower than that of ex vivo iron assays.

Paper Details

Date Published: 27 May 2016
PDF: 5 pages
J. Biomed. Opt. 21(5) 056008 doi: 10.1117/1.JBO.21.5.056008
Published in: Journal of Biomedical Optics Volume 21, Issue 5
Show Author Affiliations
Federica Maccarinelli, Univ. degli Studi di Brescia (Italy)
Fernando Carmona, Univ. degli Studi di Brescia (Italy)
Maria Regoni, Univ. degli Studi di Brescia (Italy)
Paolo Arosio, Univ. degli Studi di Brescia (Italy)

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