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Journal of Biomedical Optics • Open Access • new

Division of focal plane polarimeter-based 3 × 4 Mueller matrix microscope: a potential tool for quick diagnosis of human carcinoma tissues
Author(s): Jintao Chang; Honghui He; Ye Wang; Yi Huang; Xianpeng Li; Chao He; Ran Liao; Nan Zeng; Shaoxiong Liu; Hui Ma

Paper Abstract

A polarization microscope is a useful tool to reveal the optical anisotropic nature of a specimen and can provide abundant microstructural information about samples. We present a division of focal plane (DoFP) polarimeter-based polarization microscope capable of simultaneously measuring both the Stokes vector and the 3×4 Mueller matrix with an optimal polarization illumination scheme. The Mueller matrix images of unstained human carcinoma tissue slices show that the m24 and m34 elements can provide important information for pathological observations. The characteristic features of the m24 and m34 elements can be enhanced by polarization staining under illumination by a circularly polarized light. Hence, combined with a graphics processing unit acceleration algorithm, the DoFP polarization microscope is capable of real-time polarization imaging for potential quick clinical diagnoses of both standard and frozen slices of human carcinoma tissues.

Paper Details

Date Published: 9 May 2016
PDF: 8 pages
J. Biomed. Opt. 21(5) 056002 doi: 10.1117/1.JBO.21.5.056002
Published in: Journal of Biomedical Optics Volume 21, Issue 5
Show Author Affiliations
Jintao Chang, Graduate School at Shenzhen, Tsinghua Univ. (China)
Honghui He, Graduate School at Shenzhen, Tsinghua Univ. (China)
Ye Wang, Graduate School at Shenzhen, Tsinghua Univ. (China)
Yi Huang, Tsinghua Univ. (China)
Xianpeng Li, Graduate School at Shenzhen, Tsinghua Univ. (China)
Chao He, Graduate School at Shenzhen, Tsinghua Univ. (China)
Ran Liao, Graduate School at Shenzhen, Tsinghua Univ. (China)
Nan Zeng, Graduate School at Shenzhen, Tsinghua Univ. (China)
Shaoxiong Liu, Huazhong Univ. of Science and Technology (China)
Hui Ma, Graduate School at Shenzhen, Tsinghua Univ. (China)


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