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Journal of Biomedical Optics

Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser
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Paper Abstract

The use of small particles has expanded the capability of ultrashort pulsed laser optoinjection technology toward simultaneous treatment of multiple cells. The microfluidic platform is one of the attractive systems that has obtained synergy with laser-based technology for cell manipulation, including optoinjection. We have demonstrated the delivery of molecules into suspended-flowing cells in a microfluidic channel by using biodegradable polymer microspheres and a near-infrared femtosecond laser pulse. The use of polylactic-co-glycolic acid microspheres realized not only a higher optoinjection ratio compared to that with polylactic acid microspheres but also avoids optical damage to the microfluidic chip, which is attributable to its higher optical intensity enhancement at the localized spot under a microsphere. Interestingly, optoinjection ratios to nucleus showed a difference for adhered cells and suspended cells. The use of biodegradable polymer microspheres provides high throughput optoinjection; i.e., multiple cells can be treated in a short time, which is promising for various applications in cell analysis, drug delivery, and <italic<ex vivo</italic< gene transfection to bone marrow cells and stem cells without concerns about residual microspheres.

Paper Details

Date Published: 9 May 2016
PDF: 6 pages
J. Biomed. Opt. 21(5) 055001 doi: 10.1117/1.JBO.21.5.055001
Published in: Journal of Biomedical Optics Volume 21, Issue 5
Show Author Affiliations
Atsuhiro Ishii, Keio Univ. (Japan)
Kazumasa Ariyasu, Keio Univ. (Japan)
Tatsuki Mitsuhashi, Keio Univ. (Japan)
Dag Heinemann, Laser Zentrum Hannover e.V. (Germany)
Alexander Heisterkamp, Laser Zentrum Hannover e.V. (Germany)
Gottfried Wilhelm Leibniz Univ. Hannover (Germany)
Mitsuhiro Terakawa, Keio Univ. (Japan)


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