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Journal of Biomedical Optics • Open Access

Hyperspectral multiplex single-particle tracking of different receptor subtypes labeled with quantum dots in live neurons
Author(s): Simon Labrecque; Jean-Philippe Sylvestre; Stephane Marcet; Francesca Mangiarini; Brice Bourgoin; Marc Verhaegen; Sébastien Blais-Ouellette; Paul De Koninck

Paper Abstract

The efficacy of existing therapies and the discovery of innovative treatments for central nervous system (CNS) diseases have been limited by the lack of appropriate methods to investigate complex molecular processes at the synaptic level. To improve our capability to investigate complex mechanisms of synaptic signaling and remodeling, we designed a fluorescence hyperspectral imaging platform to simultaneously track different subtypes of individual neurotransmitter receptors trafficking in and out of synapses. This imaging platform allows simultaneous image acquisition of at least five fluorescent markers in living neurons with a high-spatial resolution. We used quantum dots emitting at different wavelengths and functionalized to specifically bind to single receptors on the membrane of living neurons. The hyperspectral imaging platform enabled the simultaneous optical tracking of five different synaptic proteins, including subtypes of glutamate receptors (mGluR and AMPAR) and postsynaptic signaling proteins. It also permitted the quantification of their mobility after treatments with various pharmacological agents. This technique provides an efficient method to monitor several synaptic proteins at the same time, which could accelerate the screening of effective compounds for treatment of CNS disorders.

Paper Details

Date Published: 25 April 2016
PDF: 11 pages
J. Biomed. Opt. 21(4) 046008 doi: 10.1117/1.JBO.21.4.046008
Published in: Journal of Biomedical Optics Volume 21, Issue 4
Show Author Affiliations
Simon Labrecque, Institut Univ. en Santé Mentale de Québec (Canada)
Jean-Philippe Sylvestre, Photon etc. Inc. (Canada)
Stephane Marcet, Photon etc. Inc. (Canada)
Francesca Mangiarini, Photon etc. Inc. (Canada)
Brice Bourgoin, Photon etc. Inc. (Canada)
Marc Verhaegen, Photon etc. Inc. (Canada)
Sébastien Blais-Ouellette, Photon etc. Inc. (Canada)
Paul De Koninck, Institut Univ. en Santé Mentale de Québec (Canada)
Ctr. de Recherche de l'Univ. Laval Robert-Giffard (Canada)


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