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Journal of Biomedical Optics

Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy
Author(s): Alba Alfonso-García; Tim D. Smith; Rupsa Datta; Thuy U. Luu; Enrico Gratton; Eric O. Potma; Wendy F. Liu
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Paper Abstract

Macrophages adopt a variety of phenotypes that are a reflection of the many functions they perform as part of the immune system. In particular, metabolism is a phenotypic trait that differs between classically activated, proinflammatory macrophages, and alternatively activated, prohealing macrophages. Inflammatory macrophages have a metabolism based on glycolysis while alternatively activated macrophages generally rely on oxidative phosphorylation to generate chemical energy. We employ this shift in metabolism as an endogenous marker to identify the phenotype of individual macrophages via live-cell fluorescence lifetime imaging microscopy (FLIM). We demonstrate that polarized macrophages can be readily discriminated with the aid of a phasor approach to FLIM, which provides a fast and model-free method for analyzing fluorescence lifetime images.

Paper Details

Date Published: 18 April 2016
PDF: 7 pages
J. Biomed. Opt. 21(4) 046005 doi: 10.1117/1.JBO.21.4.046005
Published in: Journal of Biomedical Optics Volume 21, Issue 4
Show Author Affiliations
Alba Alfonso-García, Univ. of California, Irvine (United States)
Tim D. Smith, Univ. of California, Irvine (United States)
Rupsa Datta, Univ. of California, Irvine (United States)
Thuy U. Luu, Univ. of California, Irvine (United States)
Enrico Gratton, Univ. of California, Irvine (United States)
Eric O. Potma, Univ. of California, Irvine (United States)
Wendy F. Liu, Univ. of California, Irvine (United States)

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