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Journal of Biomedical Optics

Quantifying creatinine and urea in human urine through Raman spectroscopy aiming at diagnosis of kidney disease
Author(s): Cassiano Junior Saatkamp; Maurício Liberal de Almeida; Jeyse Aliana Martins Bispo; Antonio Luiz Barbosa Pinheiro; Adriana Barrinha Fernandes; Landulfo Silveira
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Paper Abstract

Due to their importance in the regulation of metabolites, the kidneys need continuous monitoring to check for correct functioning, mainly by urea and creatinine urinalysis. This study aimed to develop a model to estimate the concentrations of urea and creatinine in urine by means of Raman spectroscopy (RS) that could be used to diagnose kidney disease. Midstream urine samples were obtained from 54 volunteers with no kidney complaints. Samples were subjected to a standard colorimetric assay of urea and creatinine and submitted to spectroscopic analysis by means of a dispersive Raman spectrometer (830 nm, 350 mW, 30 s). The Raman spectra of urine showed peaks related mainly to urea and creatinine. Partial least squares models were developed using selected Raman bands related to urea and creatinine and the biochemical concentrations in urine measured by the colorimetric method, resulting in r=0.90 and 0.91 for urea and creatinine, respectively, with root mean square error of cross-validation (RMSEcv) of 312 and 25.2  mg/dL, respectively. RS may become a technique for rapid urinalysis, with concentration errors suitable for population screening aimed at the prevention of renal diseases.

Paper Details

Date Published: 1 March 2016
PDF: 7 pages
J. Biomed. Opt. 21(3) 037001 doi: 10.1117/1.JBO.21.3.037001
Published in: Journal of Biomedical Optics Volume 21, Issue 3
Show Author Affiliations
Cassiano Junior Saatkamp, IESPES (Brazil)
Maurício Liberal de Almeida, Instituto Esperança de Ensino Superior (Brazil)
Jeyse Aliana Martins Bispo, Faculdades Integradas do Tapajós (Brazil)
Antonio Luiz Barbosa Pinheiro, Univ. Camilo Castelo Branco (Brazil)
Univ. Federal da Bahia (Brazil)
Adriana Barrinha Fernandes, Univ. Camilo Castelo Branco (Brazil)
Landulfo Silveira, Univ. Camilo Castelo Branco (Brazil)


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