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Journal of Biomedical Optics

In vivo reflectance-mode confocal microscopy assessments: impact of overweight on human skin microcirculation and histomorphology
Author(s): Ahmet A. Altintas; Matthias C. Aust; Robert Krämer; Peter M. Vogt; Mehmet Ali Altintas
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Paper Abstract

Reflectance-mode confocal microscopy (RCM) enables in vivo assessment of the human skin. Impact of overweight on both human skin microcirculation and histomorphology has not been investigated in vivo. The purpose of this study is to evaluate both microcirculation and histomorphology in vivo in overweight. In 10 normotensive overweight nondiabetic individuals (OW-group, BMI 29.1±2.7  kg/m2) and 10 age- and sex-matched healthy lean controls (CO-group, BMI 20.4±1.9  kg/m2) the following parameters were evaluated using RCM: dermal blood cell flow (DBCF), density of dermal capillaries (DDC), epidermal thickness (ET), and epidermal cell size (ECS). DBCF was counted at 63.11±4.14  cells/min in OW-group and at 51.06±3.84  cells/min in CO-group (P<0.05). DDC was reduced in OW-group (4.91±0.39  capillaries/mm2) compared to the controls (6.02±0.64  capillaries/mm2, P<0.05). Histometric evaluation of ET reveals thickening in OW-group compared to the CO-group (54.79±4.25  μm versus 44.03±3.11  μm, P<0.05). ECS differed significantly (P<0.05) in OW-group (821.3±42.02  μm2) compared to the controls (772.6±34.79  μm2). Inverse correlation of dermal capillary density and overweight point to reduced total tissue perfusion while positive related blood cell flow reveals vasodilatation. Increase of both ET and cell size indicates remodeling of cutaneous histomorphology, maybe as an early stage of adiposity-related skin condition.

Paper Details

Date Published: 28 March 2016
PDF: 5 pages
J. Biomed. Opt. 21(3) 036009 doi: 10.1117/1.JBO.21.3.036009
Published in: Journal of Biomedical Optics Volume 21, Issue 3
Show Author Affiliations
Ahmet A. Altintas, Medical Ctr. Cologne (Germany)
Matthias C. Aust, Johanniter-Kliniken Bonn (Germany)
Robert Krämer, Universitätklinikum Schleswig-Holstein (Germany)
Peter M. Vogt, Medizinische Hochschule Hannover (Germany)
Mehmet Ali Altintas, Bergmannsheil Buer (Germany)

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