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Journal of Biomedical Optics

Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain
Author(s): Mélanie Pedrazzani; Vincent Loriette; Paul Tchenio; Sakina Benrezzak; Daniele Nutarelli; Alexandra Fragola
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Paper Abstract

We present an implementation of a sensorless adaptive optics loop in a widefield fluorescence microscope. This setup is designed to compensate for aberrations induced by the sample on both excitation and emission pathways. It allows fast optical sectioning inside a living Drosophila brain. We present a detailed characterization of the system performances. We prove that the gain brought to optical sectioning by realizing structured illumination microscopy with adaptive optics down to 50  μm deep inside living Drosophila brain.

Paper Details

Date Published: 11 March 2016
PDF: 9 pages
J. Biomed. Opt. 21(3) 036006 doi: 10.1117/1.JBO.21.3.036006
Published in: Journal of Biomedical Optics Volume 21, Issue 3
Show Author Affiliations
Mélanie Pedrazzani, Lab. Aimé Cotton (France)
Vincent Loriette, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)
Paul Tchenio, Lab. Aimé Cotton (France)
Ctr. National de la Recherche Scientifique (France)
Sakina Benrezzak, Lab. Aimé Cotton (France)
Daniele Nutarelli, Lab. Aimé Cotton (France)
Alexandra Fragola, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)

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