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Journal of Biomedical Optics • Open Access

Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Paper Abstract

Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under <italic<in vitro</italic< conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.

Paper Details

Date Published: 8 March 2016
PDF: 10 pages
J. Biomed. Opt. 21(3) 035001 doi: 10.1117/1.JBO.21.3.035001
Published in: Journal of Biomedical Optics Volume 21, Issue 3
Show Author Affiliations
Kisung Lee, Univ. of Oulu (Finland)
M.V. Lomonosov Moscow SU (Russian Federation)
Matti Kinnunen, Univ. of Oulu (Finland)
Maria D. Khokhlova, M.V. Lomonosov Moscow SU (Russia)
Evgeny V. Lyubin, M.V. Lomonosov Moscow SU (Russia)
Alexander V. Priezzhev, M.V. Lomonosov Moscow SU (Russian Federation)
Igor Meglinski, Univ. of Oulu (Finland)
Andrey A. Fedyanin, M.V. Lomonosov Moscow SU (Russia)

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