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Journal of Biomedical Optics • Open Access

Quantitative performance characterization of three-dimensional noncontact fluorescence molecular tomography
Author(s): Rosy Favicchio; Stylianos Psycharakis; Kai Schönig; Dusan Bartsch; Clio Mamalaki; Joseph Papamatheakis; Jorge Ripoll; Giannis Zacharakis

Paper Abstract

Fluorescent proteins and dyes are routine tools for biological research to describe the behavior of genes, proteins, and cells, as well as more complex physiological dynamics such as vessel permeability and pharmacokinetics. The use of these probes in whole body <italic<in vivo</italic< imaging would allow extending the range and scope of current biomedical applications and would be of great interest. In order to comply with a wide variety of application demands, <italic<in vivo</italic< imaging platform requirements span from wide spectral coverage to precise quantification capabilities. Fluorescence molecular tomography (FMT) detects and reconstructs in three dimensions the distribution of a fluorophore <italic<in vivo</italic<. Noncontact FMT allows fast scanning of an excitation source and noninvasive measurement of emitted fluorescent light using a virtual array detector operating in free space. Here, a rigorous process is defined that fully characterizes the performance of a custom-built horizontal noncontact FMT setup. Dynamic range, sensitivity, and quantitative accuracy across the visible spectrum were evaluated using fluorophores with emissions between 520 and 660 nm. These results demonstrate that high-performance quantitative three-dimensional visible light FMT allowed the detection of challenging mesenteric lymph nodes <italic<in vivo</italic< and the comparison of spectrally distinct fluorescent reporters in cell culture.

Paper Details

Date Published: 18 February 2016
PDF: 10 pages
J. Biomed. Opt. 21(2) 026009 doi: 10.1117/1.JBO.21.2.026009
Published in: Journal of Biomedical Optics Volume 21, Issue 2
Show Author Affiliations
Rosy Favicchio, Imperial College Healthcare NHS Trust (United Kingdom)
Foundation for Research and Technology Hellas (Greece)
Stylianos Psycharakis, Foundation for Research and Technology-Hellas (Greece)
Kai Schönig, Ruprecht-Karls-Univ. Heidelberg (Germany)
Central Institute of Mental Health (Germany)
Dusan Bartsch, Ruprecht-Karls-Univ. Heidelberg (Germany)
Central Institute of Mental Health (Germany)
Clio Mamalaki, Foundation for Research and Technology-Hellas (Greece)
Joseph Papamatheakis, Foundation for Research and Technology-Hellas (Greece)
Jorge Ripoll, Univ. Carlos III de Madrid (Spain)
Instituto de Investigación Sanitaria del Hospital Gregorio Marañón (Spain)
Giannis Zacharakis, Foundation for Research and Technology-Hellas (Greece)

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