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Journal of Biomedical Optics • Open Access

Wide field of view multifocal scanning microscopy with sparse sampling
Author(s): Jie Wang; Jigang Wu

Paper Abstract

We propose to use sparsely sampled line scans with a sparsity-based reconstruction method to obtain images in a wide field of view (WFOV) multifocal scanning microscope. In the WFOV microscope, we used a holographically generated irregular focus grid to scan the sample in one dimension and then reconstructed the sample image from line scans by measuring the transmission of the foci through the sample during scanning. The line scans were randomly spaced with average spacing larger than the Nyquist sampling requirement, and the image was recovered with sparsity-based reconstruction techniques. With this scheme, the acquisition data can be significantly reduced and the restriction for equally spaced foci positions can be removed, indicating simpler experimental requirement. We built a prototype system and demonstrated the effectiveness of the reconstruction by recovering microscopic images of a U.S. Air Force target and an onion skin cell microscope slide with 40, 60, and 80% missing data with respect to the Nyquist sampling requirement.

Paper Details

Date Published: 17 February 2016
PDF: 6 pages
J. Biomed. Opt. 21(2) 026008 doi: 10.1117/1.JBO.21.2.026008
Published in: Journal of Biomedical Optics Volume 21, Issue 2
Show Author Affiliations
Jie Wang, Shanghai Jiao Tong Univ. (China)
Jigang Wu, Shanghai Jiao Tong Univ. (China)

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