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Journal of Biomedical Optics

Rotational imaging optical coherence tomography for full-body mouse embryonic imaging
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Paper Abstract

Optical coherence tomography (OCT) has been widely used to study mammalian embryonic development with the advantages of high spatial and temporal resolutions and without the need for any contrast enhancement probes. However, the limited imaging depth of traditional OCT might prohibit visualization of the full embryonic body. To overcome this limitation, we have developed a new methodology to enhance the imaging range of OCT in embryonic day (E) 9.5 and 10.5 mouse embryos using rotational imaging. Rotational imaging OCT (RI-OCT) enables full-body imaging of mouse embryos by performing multiangle imaging. A series of postprocessing procedures was performed on each cross-section image, resulting in the final composited image. The results demonstrate that RI-OCT is able to improve the visualization of internal mouse embryo structures as compared to conventional OCT.

Paper Details

Date Published: 5 February 2016
PDF: 9 pages
J. Biomed. Opt. 21(2) 026002 doi: 10.1117/1.JBO.21.2.026002
Published in: Journal of Biomedical Optics Volume 21, Issue 2
Show Author Affiliations
Chen Wu, Univ. of Houston (United States)
Narendran Sudheendran, Univ. of Houston (United States)
Manmohan Singh, Univ. of Houston (United States)
Irina V. Larina, Baylor College of Medicine (United States)
Mary E. Dickinson, Baylor College of Medicine (United States)
Kirill V. Larin, Univ. of Houston (United States)
Baylor College of Medicine (United States)
Tomsk State Univ. (Russia)

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