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Journal of Biomedical Optics

Structural effects of simvastatin on rat liver tissue: Fourier transform infrared and Raman microspectroscopic studies
Author(s): Sebnem Garip; Sevgi Haman Bayari; Mete Severcan; Sherif Abbas; Igor K. Lednev; Feride Severcan
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Paper Abstract

Simvastatin is one of the most frequently prescribed statins because of its efficacy in the treatment of hypercholesterolemia, reducing cardiovascular risk and related mortality. Determination of its side effects on different tissues is mandatory to improve safe use of this drug. In the present study, the effects of simvastatin on molecular composition and structure of healthy rat livers were investigated by Fourier transform infrared and Raman imaging. Simvastatin-treated groups received 50  mg/kg/day simvastatin for 30 days. The ratio of the area and/or intensity of the bands assigned to lipids, proteins, and nucleic acids were calculated to get information about the drug-induced changes in tissues. Loss of unsaturation, accumulation of end products of lipid peroxidation, and alterations in lipid-to-protein ratio were observed in the treated group. Protein secondary structure studies revealed significant decrease in α-helix and increase in random coil, while native β-sheet decreases and aggregated β-sheet increases in treated group implying simvastatin-induced protein denaturation. Moreover, groups were successfully discriminated using principal component analysis. Consequently, high-dose simvastatin treatment induces hepatic lipid peroxidation and changes in molecular content and protein secondary structure, implying the risk of liver disorders in drug therapy.

Paper Details

Date Published: 18 February 2016
PDF: 13 pages
J. Biomed. Opt. 21(2) 025008 doi: 10.1117/1.JBO.21.2.025008
Published in: Journal of Biomedical Optics Volume 21, Issue 2
Show Author Affiliations
Sebnem Garip, Istanbul Kemerburgaz Univ. (Turkey)
Sevgi Haman Bayari, Hacettepe Univ. (Turkey)
Mete Severcan, Middle East Technical Univ. (Turkey)
Sherif Abbas, Middle East Technical University (Turkey)
Igor K. Lednev, Univ. at Albany (United States)
Feride Severcan, University at Albany (United States)


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