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Journal of Biomedical Optics • Open Access

Toward noninvasive assessment of flap viability with time-resolved diffuse optical tomography: a preclinical test on rats
Author(s): Laura Di Sieno; Georges Bettega; Michel Berger; Cynthia Hamou; Marion Aribert; Alberto Dalla Mora; Agathe Puszka; Henri Grateau; Davide Contini; Lionel Hervé; Jean-Luc Coll; Jean-Marc Dinten; Antonio Pifferi; Anne Planat-Chrétien

Paper Abstract

The noninvasive assessment of flap viability in autologous reconstruction surgery is still an unmet clinical need. To cope with this problem, we developed a proof-of-principle fully automatized setup for fast time-gated diffuse optical tomography exploiting Mellin–Laplace transform to obtain three-dimensional tomographic reconstructions of oxy- and deoxy-hemoglobin concentrations. We applied this method to perform preclinical tests on rats inducing total venous occlusion in the cutaneous abdominal flaps. Notwithstanding the use of just four source-detector couples, we could detect a spatially localized increase of deoxyhemoglobin following the occlusion (up to 550  μM in 54 min). Such capability to image spatio-temporal evolution of blood perfusion is a key issue for the noninvasive monitoring of flap viability.

Paper Details

Date Published: 2 February 2016
PDF: 8 pages
J. Biomed. Opt. 21(2) 025004 doi: 10.1117/1.JBO.21.2.025004
Published in: Journal of Biomedical Optics Volume 21, Issue 2
Show Author Affiliations
Laura Di Sieno, Politecnico di Milano (Italy)
Georges Bettega, Hôpital d’Annecy (France)
CHU Grenoble (France)
Univ. Grenoble Alpes (France)
Michel Berger, Commissariat à l'Énergie Atomique (France)
Cynthia Hamou, University Grenoble Alpes-site santé (France)
University Grenoble Alpes (France)
Centre Hospitalier Universitaire–Grenoble (France)
Marion Aribert, CHU Grenoble (France)
Univ. Grenoble Alpes (France)
Alberto Dalla Mora, Politecnico di Milano (Italy)
Agathe Puszka, CHU Grenoble (France)
Univ. Grenoble Alpes (France)
CEA-LETI (France)
Henri Grateau, Commissariat à l'Énergie Atomique (France)
Davide Contini, Politecnico di Milano (Italy)
Lionel Hervé, MINATEC (France)
Jean-Luc Coll, University Grenoble Alpes-site santé (France)
University Grenoble Alpes (France)
Jean-Marc Dinten, CEA-LETI (France)
Antonio Pifferi, Politecnico di Milano (Italy)
Istituto di Fotonica e Nanotecnologie (Italy)
Anne Planat-Chrétien, CEA-LETI (France)

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