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Journal of Biomedical Optics

Fast spectral surface plasmon resonance imaging sensor for real-time high-throughput detection of biomolecular interactions
Author(s): Kaiqiang Chen; Youjun Zeng; Lei Wang; Dayong Gu; Jianan He; Shu-Yuen Wu; Ho-Pui Ho; Xuejin Li; Junle Qu; Bruce Zhi Gao; Yonghong Shao
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Paper Abstract

A fast surface plasmon resonance (SPR) imaging biosensor system based on wavelength interrogation using a liquid crystal tunable filter (LCTF) is presented. The system combines the merits of wide-dynamic detection range offered by the spectral approach and multiplexed high-throughput data collection with a two-dimensional (2-D) biosensor array. The key feature of the reported scheme is a feedback loop that drives the LCTF to achieve fast tracking of the SPR dip movement caused by the binding of target molecules to the sensor surface. Experimental results show that the system is capable of completing an SPR dip measurement within 4 s. Based on using a spectral window of about 100 nm, the experimental dynamic detection range and refractive index resolution are 4.63×10−2  RIU and 5.87×10−6  RIU, respectively. As also demonstrated herein using 2-D microsensor arrays, among the spectral SPR sensors, the reported system is most suitable for multiplexed label-free biosensing applications.

Paper Details

Date Published: 9 December 2016
PDF: 7 pages
J. Biomed. Opt. 21(12) 127003 doi: 10.1117/1.JBO.21.12.127003
Published in: Journal of Biomedical Optics Volume 21, Issue 12
Show Author Affiliations
Kaiqiang Chen, Shenzhen Univ. (China)
Youjun Zeng, Shenzhen Univ. (China)
Lei Wang, Shenzhen Univ. (China)
Dayong Gu, Shenzhen Entry-Exit Inspection and Quarantine Bureau (China)
Jianan He, Shenzhen Entry-Exit Inspection and Quarantine Bureau (China)
Shu-Yuen Wu, The Chinese Univ. of Hong Kong (Hong Kong, China)
Ho-Pui Ho, The Chinese Univ. of Hong Kong (Hong Kong, China)
Xuejin Li, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Bruce Zhi Gao, Clemson Univ. (United States)
Yonghong Shao, Shenzhen Univ. (China)

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