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Journal of Biomedical Optics • Open Access

High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator
Author(s): Séverine Coquoz; Amir Nahas; Miguel Sison; Antonio Lopez; Theo Lasser

Paper Abstract

We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy with technical samples, we demonstrate the high acquisition rate while monitoring cellular processes at temporal scales ranging from milliseconds to hours.

Paper Details

Date Published: 23 December 2016
PDF: 5 pages
J. Biomed. Opt. 21(12) 126019 doi: 10.1117/1.JBO.21.12.126019
Published in: Journal of Biomedical Optics Volume 21, Issue 12
Show Author Affiliations
Séverine Coquoz, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Amir Nahas, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Miguel Sison, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Antonio Lopez, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Theo Lasser, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


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