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Journal of Biomedical Optics

Self-guided reconstruction for time-domain fluorescence molecular lifetime tomography
Author(s): Chuangjian Cai; Wenjuan Cai; Jiaju Cheng; Yuxuan Yang; Jianwen Luo
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Paper Abstract

Fluorescence probes have distinct yields and lifetimes when located in different environments, which makes the reconstruction of fluorescence molecular lifetime tomography (FMLT) challenging. To enhance the reconstruction performance of time-domain (TD) FMLT with heterogeneous targets, a self-guided L 1 regularization projected steepest descent (SGL1PSD) algorithm is proposed. Different from other algorithms performed in time domain, SGL1PSD introduces a time-resolved strategy into fluorescence yield reconstruction. The algorithm consists of four steps. Step 1 reconstructs the initial yield map with full time gate strategy; steps 2–4 reconstruct the inverse lifetime map, the yield map, and the inverse lifetime map again with time-resolved strategy, respectively. The reconstruction result of each step is used as a priori for the reconstruction of the next step. Projected iterated Tikhonov regularization algorithm is adopted for the yield map reconstructions in steps 1 and 3 to provide a solution with iterative refinement and nonnegative constraint. The inverse lifetime map reconstructions in steps 2 and 4 are based on L 1 regularization projected steepest descent algorithm, which employ the L 1 regularization to reduce the ill-posedness of the high-dimensional nonlinear problem. Phantom experiments with heterogeneous targets at different edge-to-edge distances demonstrate that SGL1PSD can provide high resolution and quantification accuracy for TD FMLT.

Paper Details

Date Published: 21 December 2016
PDF: 10 pages
J. Biomed. Opt. 21(12) 126012 doi: 10.1117/1.JBO.21.12.126012
Published in: Journal of Biomedical Optics Volume 21, Issue 12
Show Author Affiliations
Chuangjian Cai, Tsinghua Univ. (China)
Wenjuan Cai, Tsinghua Univ. (China)
Jiaju Cheng, Tsinghua Univ. School of Medicine (China)
Yuxuan Yang, Tsinghua Univ. School of Medicine (China)
Jianwen Luo, Tsinghua Univ. (China)

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