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Journal of Biomedical Optics • Open Access

Low-cost multimodal light sheet microscopy for optically cleared tissues and living specimens
Author(s): Vincent Rouger; Ricardo Alchini; Alexei Kazarine; Angelica A. Gopal; Marie-Pier Girouard; Alyson E. Fournier; Paul W. Wiseman

Paper Abstract

Light sheet microscopy techniques have expanded with designs to address many new applications. Due to rapid advancements in computing power, camera/detector technologies, and tissue clearing techniques, light sheet methods are becoming increasingly popular for biomedical imaging applications at the cellular and tissue levels. Light sheet imaging modalities couple rapid imaging rates, low-levels of phototoxicity, and excellent signal to noise ratios, contributing to their popularity for experimental biology. However, the current major limitation of light sheet microscopy arises from optical aberrations, with the main drawback being the defocusing introduced by refractive index variations that accompany clearing techniques. Here, we propose an inexpensive and easy to build light sheet based instrumentation to overcome this limitation by optomechanically decoupling the sample scanning movement from the detection step. Our solution is relatively simple to implement and also provides increased modularity by using a swappable excitation arm. This expands the range of samples we can image on a single system, from high resolution for single cells at μ m spatial resolution, to tissues with mm spatial resolution. We demonstrate our approach, using the system to image iDISCO cleared embryos and sciatic nerves, and provide the full three-dimensional reconstruction of these objects in minutes.

Paper Details

Date Published: 20 December 2016
PDF: 10 pages
J. Biomed. Opt. 21(12) 126008 doi: 10.1117/1.JBO.21.12.126008
Published in: Journal of Biomedical Optics Volume 21, Issue 12
Show Author Affiliations
Vincent Rouger, McGill Univ. (Canada)
Ricardo Alchini, McGill Univ. (Canada)
Alexei Kazarine, McGill Univ. (Canada)
Angelica A. Gopal, McGill Univ. (Canada)
Marie-Pier Girouard, McGill Univ. (Canada)
Alyson E. Fournier, McGill Univ. (Canada)
Paul W. Wiseman, McGill Univ. (Canada)

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