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Journal of Biomedical Optics

Optimization and characterization of the endogenous production of protoporphyrin IX in a yeast model
Author(s): Jaroslava Joniová; Emmanuel Gerelli; Matthieu Zellweger; Georges Wagnières
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Paper Abstract

The availability of reproducible, convenient, and inexpensive model organisms able to generate predictable levels of endogenous porphyrins, including protoporphyrin IX (PpIX), is essential in photomedicine research. Saccharomyces cerevisiae produces endogenous PpIX and was used as a model organism for this study with the aim to maximize endogenous PpIX fluorescence intensity. It was found that PpIX fluorescence was significantly enhanced by administration of 5-aminolevulinic acid (ALA) and 2,2′-bipyridyl. Fluorescence intensity and spectroscopy of PpIX produced endogenously were measured in diluted yeast solutions under various conditions. The optimal protocol was: 5    μ M ALA and 1 mM 2,2′-bipyridyl administered synchronously at 32°C. After 3 h, PpIX in yeast demonstrated similar steady-state and time-resolved spectroscopy as that of PpIX in DMSO. Moreover, under hypoxic conditions, the reciprocal lifetime of PpIX delayed fluorescence measured in real time was correlated to the partial pressure of oxygen ( pO 2 ) measured concomitantly with a commercially available pO 2 probe. These data show that yeast can, in optimal conditions, reproducibly generate PpIX. This is of interest in various fields such as photodiagnosis, photodynamic therapy, and photobiomodulation. Use of this model organism focuses on essential mechanisms, without the complexity of a multicellular organism.

Paper Details

Date Published: 21 December 2016
PDF: 8 pages
J. Biomed. Opt. 21(12) 125008 doi: 10.1117/1.JBO.21.12.125008
Published in: Journal of Biomedical Optics Volume 21, Issue 12
Show Author Affiliations
Jaroslava Joniová, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Emmanuel Gerelli, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Matthieu Zellweger, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Georges Wagnières, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

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