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Journal of Biomedical Optics • Open Access

Development of a laser-activated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research
Author(s): Lien M. Davidson; Natalia Barkalina; Marc Yeste; Celine Jones; Kevin Coward

Paper Abstract

Nanoparticles have revolutionized medical research over the last decade. One notable emerging area of nanomedicine is research developments in the reproductive sciences. Since increasing evidence indicates links between abnormal gene expression and previously unexplained states of infertility, there is a strong impetus to develop tools, such as nanoparticle platforms, to elucidate the pathophysiological mechanisms underlying such states. Mesoporous silica nanoparticles (MSNPs) represent a powerful and safe delivery tool for molecular and genetic investigations. Nevertheless, ongoing progress is restricted by low efficiency and unpredictable control of cargo delivery. Here, we describe for the first time, the development of a laser-activated MSNP system with heat-responsive cargo. Data derived from human embryonic kidney cells (HEK293T) indicate that when driven by a heat-shock promoter, MSNP cargo exhibits a significantly increased expression following infrared laser stimulus to stimulate a heat-shock response, without adverse cytotoxic effects. This delivery platform, with increased efficiency and the ability to impart spatial and temporal control, is highly useful for molecular and genetic investigations. We envision that this straightforward stimuli-responsive system could play a significant role in developing efficient nanodevices for research applications, for example in reproductive medicine.

Paper Details

Date Published: 14 November 2016
PDF: 9 pages
J. Biomed. Opt. 21(11) 115002 doi: 10.1117/1.JBO.21.11.115002
Published in: Journal of Biomedical Optics Volume 21, Issue 11
Show Author Affiliations
Lien M. Davidson, Univ. of Oxford (United Kingdom)
Natalia Barkalina, Univ. of Oxford (United Kingdom)
Marc Yeste, Univ. of Oxford (United Kingdom)
Celine Jones, Univ. of Oxford (United Kingdom)
Kevin Coward, Univ. of Oxford (United Kingdom)

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