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Journal of Biomedical Optics

Reflected scatterometry for noninvasive interrogation of bacterial colonies
Author(s): Huisung Kim; Iyll-Joon Doh; Jennifer Sturgis; Arun K. Bhunia; J. Paul Robinson; Euiwon Bae
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Paper Abstract

A phenotyping of bacterial colonies on agar plates using forward-scattering diffraction-pattern analysis provided promising classification of several different bacteria such as Salmonella, Vibrio, Listeria, and E. coli. Since the technique is based on forward-scattering phenomena, light transmittance of both the colony and the medium is critical to ensure quality data. However, numerous microorganisms and their growth media allow only limited light penetration and render the forward-scattering measurement a challenging task. For example, yeast, Lactobacillus, mold, and several soil bacteria form colorful and dense colonies that obstruct most of the incoming light passing through them. Moreover, blood agar, which is widely utilized in the clinical field, completely blocks the incident coherent light source used in forward scatterometry. We present a newly designed reflection scatterometer and validation of the resolving power of the instrument. The reflectance-type instrument can acquire backward elastic scatter patterns for both highly opaque media and colonies and has been tested with three different bacterial genera grown on blood agar plates. Cross-validation results show a classification rate above 90% for four genera.

Paper Details

Date Published: 24 October 2016
PDF: 9 pages
J. Biomed. Opt. 21(10) 107004 doi: 10.1117/1.JBO.21.10.107004
Published in: Journal of Biomedical Optics Volume 21, Issue 10
Show Author Affiliations
Huisung Kim, Purdue Univ. (United States)
Iyll-Joon Doh, Purdue Univ. (United States)
Jennifer Sturgis, Purdue Univ. (United States)
Arun K. Bhunia, Purdue Univ. (United States)
J. Paul Robinson, Purdue Univ. (United States)
Euiwon Bae, Purdue Univ. (United States)

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