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Journal of Biomedical Optics

Quantitative phase imaging of live cells with near on-axis digital holographic microscopy using constrained optimization approach
Author(s): Vimal Prabhu Pandiyan; Kedar Khare; Renu John
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Paper Abstract

We demonstrate a single-shot near on-axis digital holographic microscope that uses a constrained optimization approach for retrieval of the complex object function in the hologram plane. The recovered complex object is back-propagated from the hologram plane to image plane using the Fresnel back-propagation algorithm. A numerical aberration compensation algorithm is employed for correcting the aberrations in the object beam. The reference beam angle is calculated automatically using the modulation property of Fourier transform without any additional recording. We demonstrate this approach using a United States Air Force (USAF) resolution target as an object on our digital holographic microscope. We also demonstrate this approach by recovering the quantitative phase images of live yeast cells, red blood cells and dynamics of live dividing yeast cells.

Paper Details

Date Published: 21 October 2016
PDF: 10 pages
J. Biomed. Opt. 21(10) 106003 doi: 10.1117/1.JBO.21.10.106003
Published in: Journal of Biomedical Optics Volume 21, Issue 10
Show Author Affiliations
Vimal Prabhu Pandiyan, Indian Institute of Technology Hyderabad (India)
Kedar Khare, Indian Institute of Technology Delhi (India)
Renu John, Indian Institute of Technology Hyderabad (India)

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