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Journal of Biomedical Optics

Multiplexed fluorescence mediated tomography with temporal and spectral data
Author(s): Ying Mu; Vivian Pera; Mark Niedre
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Paper Abstract

We recently developed an algorithm for multiplexed fluorescence tomographic imaging of at least four fluorophores concurrently in the red and near-infrared wavelength region by jointly using spectral and temporal data. We report the design of a fluorescence tomography instrument that acquires spectral and temporal data, and validate its use in tissue-mimicking phantoms with four embedded fluorescent targets with highly overlapped spectral signatures. Critically, this requires measurement or computation of extended fluorophore signature libraries, which capture the variability in the measured signal due to the unknown position of the targets in the media. We demonstrate that we can demix and tomographically image all four fluorophores with zero image cross-talk, and 1 mm or better spatial resolution.

Paper Details

Date Published: 4 October 2016
PDF: 11 pages
J. Biomed. Opt. 21(10) 105001 doi: 10.1117/1.JBO.21.10.105001
Published in: Journal of Biomedical Optics Volume 21, Issue 10
Show Author Affiliations
Ying Mu, Northeastern Univ. (United States)
Vivian Pera, Northeastern Univ. (United States)
Mark Niedre, Northeastern Univ. (United States)

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