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Journal of Biomedical Optics • Open Access • new

Ecological changes in oral microcosm biofilm during maturation
Author(s): Young-Seok Kim; Si-Mook Kang; Eun-Song Lee; Ji Hyun Lee; Bo-Ra Kim; Baek-Il Kim

Paper Abstract

The aim of this study was to evaluate the ecological changes in the biofilm at different stages of maturation using 16S rDNA gene amplicon sequencing and to identify correlations between red/green (R/G) fluorescence ratio and ecological changes. An oral microcosm biofilm was initiated from the saliva of a single donor and grown anaerobically for up to 10 days in basal medium mucin. Quantitative light-induced fluorescence analysis was shown that the R/G ratio of the biofilm increased consistently, but the slope rapidly decreased after six days. The bacterial compositions of 10 species also consistently changed over time. However, there was no significant correlation between each bacteria and red fluorescence. The monitoring of the maturation process of oral microcosm biofilm over 10 days revealed that the R/G ratio and the bacterial composition within biofilm consistently changed. Therefore, the R/G fluorescence ratio of biofilm may be related with its ecological change rather than specific bacteria.

Paper Details

Date Published: 7 March 2016
PDF: 6 pages
J. Biomed. Opt. 21(10) 101409 doi: 10.1117/1.JBO.21.10.101409
Published in: Journal of Biomedical Optics Volume 21, Issue 10
Show Author Affiliations
Young-Seok Kim, Kyungpook National Univ. (Republic of Korea)
Si-Mook Kang, Yonsei Univ. College of Dentistry (Republic of Korea)
Eun-Song Lee, Yonsei Univ. College of Dentistry (Republic of Korea)
Ji Hyun Lee, Yonsei Univ. College of Dentistry (Republic of Korea)
Bo-Ra Kim, Yonsei Univ. College of Dentistry (Republic of Korea)
Baek-Il Kim, Yonsei Univ. College of Dentistry (Republic of Korea)


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