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Journal of Biomedical Optics • Open Access

Light-sheet microscopy by confocal line scanning of dual-Bessel beams
Author(s): Pengfei Zhang; Mary E. Phipps; Peter M. Goodwin; James H. Werner

Paper Abstract

We have developed a light-sheet microscope that uses confocal scanning of dual-Bessel beams for illumination. A digital micromirror device (DMD) is placed in the intermediate image plane of the objective used to collect fluorescence and is programmed with two lines of pixels in the “on” state such that the DMD functions as a spatial filter to reject the out-of-focus background generated by the side-lobes of the Bessel beams. The optical sectioning and out-of-focus background rejection capabilities of this microscope were demonstrated by imaging of fluorescently stained actin in human A431 cells. The dual-Bessel beam system enables twice as many photons to be detected per imaging scan, which is useful for low light applications (e.g., single-molecule localization) or imaging at high speed with a superior signal to noise. While demonstrated for two Bessel beams, this approach is scalable to a larger number of beams.

Paper Details

Date Published: 25 October 2016
PDF: 4 pages
J. Biomed. Opt. 21(10) 100502 doi: 10.1117/1.JBO.21.10.100502
Published in: Journal of Biomedical Optics Volume 21, Issue 10
Show Author Affiliations
Pengfei Zhang, Washington Univ. in St. Louis (United States)
Mary E. Phipps, Los Alamos National Lab. (United States)
Peter M. Goodwin, Los Alamos National Lab. (United States)
James H. Werner, Los Alamos National Lab. (United States)


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