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Journal of Biomedical Optics

Intraoperative optical assessment of photodynamic therapy response of superficial oral squamous cell carcinoma
Author(s): Daniel J. Rohrbach; Nestor R. Rigual; Hassan Arshad; Erin C. Tracy; Michele T. Cooper; Gal Shafirstein; Gregory Wilding; Mihai Merzianu; Heinz Baumann; Barbara W. Henderson; Ulas Sunar
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Paper Abstract

This study investigated whether diffuse optical spectroscopy (DOS) measurements could assess clinical response to photodynamic therapy (PDT) in patients with head and neck squamous cell carcinoma (HNSCC). In addition, the correlation between parameters measured with DOS and the crosslinking of signal transducer and activator of transcription 3 (STAT3), a molecular marker for PDT-induced photoreaction, was investigated. Thirteen patients with early stage HNSCC received the photosensitizer 2-[1-hexyloxyethyl]-2-devinylpyropheophorbide-a (HPPH) and DOS measurements were performed before and after PDT in the operating room (OR). In addition, biopsies were acquired after PDT to assess the STAT3 crosslinking. Parameters measured with DOS, including blood volume fraction, blood oxygen saturation (StO2), HPPH concentration (cHPPH), HPPH fluorescence, and blood flow index (BFI), were compared to the pathologic response and the STAT3 crosslinking. The best individual predictor of pathological response was a change in cHPPH (sensitivity=60%, specificity=100%), while discrimination analysis using a two-parameter classifier (change in cHPPH and change in StO2) classified pathological response with 100% sensitivity and 100% specificity. BFI showed the best correlation with the crosslinking of STAT3. These results indicate that DOS-derived parameters can assess the clinical response in the OR, allowing for earlier reintervention if needed.

Paper Details

Date Published: 18 January 2016
PDF: 8 pages
J. Biomed. Opt. 21(1) 018002 doi: 10.1117/1.JBO.21.1.018002
Published in: Journal of Biomedical Optics Volume 21, Issue 1
Show Author Affiliations
Daniel J. Rohrbach, Roswell Park Cancer Institute (United States)
Wright State Univ. (United States)
Nestor R. Rigual, Roswell Park Cancer Institute (United States)
Hassan Arshad, Roswell Park Cancer Institute (United States)
Erin C. Tracy, Roswell Park Cancer Institute (United States)
Michele T. Cooper, Roswell Park Cancer Institute (United States)
Gal Shafirstein, Roswell Park Cancer Institute (United States)
Gregory Wilding, Roswell Park Cancer Institute (United States)
Mihai Merzianu, Roswell Park Cancer Institute (United States)
Heinz Baumann, Roswell Park Cancer Institute (United States)
Barbara W. Henderson, Roswell Park Cancer Institute (United States)
Ulas Sunar, Roswell Park Cancer Institute (United States)
Wright State Univ. (United States)
Univ. at Buffalo (United States)


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