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Journal of Biomedical Optics • Open Access

Multiview hyperspectral topography of tissue structural and functional characteristics

Paper Abstract

Accurate and <italic<in vivo</italic< characterization of structural, functional, and molecular characteristics of biological tissue will facilitate quantitative diagnosis, therapeutic guidance, and outcome assessment in many clinical applications, such as wound healing, cancer surgery, and organ transplantation. We introduced and tested a multiview hyperspectral imaging technique for noninvasive topographic imaging of cutaneous wound oxygenation. The technique integrated a multiview module and a hyperspectral module in a single portable unit. Four plane mirrors were cohered to form a multiview reflective mirror set with a rectangular cross section. The mirror set was placed between a hyperspectral camera and the target biological tissue. For a single image acquisition task, a hyperspectral data cube with five views was obtained. The five-view hyperspectral image consisted of a main objective image and four reflective images. Three-dimensional (3-D) topography of the scene was achieved by correlating the matching pixels between the objective image and the reflective images. 3-D mapping of tissue oxygenation was achieved using a hyperspectral oxygenation algorithm. The multiview hyperspectral imaging technique was validated in a wound model, a tissue-simulating blood phantom, and <italic<in vivo</italic< biological tissue. The experimental results demonstrated the technical feasibility of using multiview hyperspectral imaging for 3-D topography of tissue functional properties.

Paper Details

Date Published: 28 January 2016
PDF: 11 pages
J. Biomed. Opt. 21(1) 016012 doi: 10.1117/1.JBO.21.1.016012
Published in: Journal of Biomedical Optics Volume 21, Issue 1
Show Author Affiliations
Peng Liu, Univ. of Science and Technology of China (China)
Jiwei Huang, The Ohio State Univ. (United States)
Shiwu Zhang, Univ. of Science and Technology of China (China)
The Ohio State Univ. (United States)
Ronald X. Xu, The Ohio State Univ. (United States)
Univ. of Science and Technology of China (China)

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