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Journal of Biomedical Optics

Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy
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Paper Abstract

We present the first experimental demonstration of two-photon Mueller matrix second-harmonic generation (SHG) microscopy for the extraction of representative Mueller matrices. Individual SHG images of porcine sample sets are divided into subimages, and the local nonlinear two-photon Mueller matrices (M(2)) and degree of polarization (p) are determined. Results of the two-photon Mueller matrices from different sample types and the effects of thickness on the mean p distribution per polarization basis are investigated. We find that the p distribution shape changes and an associated bimodal mean difference (μd) increases with thickness. Our approach has potential as a quantitative imaging technique and can be applied to other multiphoton coherent imaging modalities.

Paper Details

Date Published: 26 January 2016
PDF: 6 pages
J. Biomed. Opt. 21(1) 016011 doi: 10.1117/1.JBO.21.1.016011
Published in: Journal of Biomedical Optics Volume 21, Issue 1
Show Author Affiliations
Chukwuemeka Okoro, Univ. of Illinois at Urbana-Champaign (United States)
Kimani C. Toussaint, Univ. of Illinois at Urbana-Champaign (United States)


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