Share Email Print
cover

Journal of Biomedical Optics

Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We present the first experimental demonstration of two-photon Mueller matrix second-harmonic generation (SHG) microscopy for the extraction of representative Mueller matrices. Individual SHG images of porcine sample sets are divided into subimages, and the local nonlinear two-photon Mueller matrices (M(2)) and degree of polarization (p) are determined. Results of the two-photon Mueller matrices from different sample types and the effects of thickness on the mean p distribution per polarization basis are investigated. We find that the p distribution shape changes and an associated bimodal mean difference (μd) increases with thickness. Our approach has potential as a quantitative imaging technique and can be applied to other multiphoton coherent imaging modalities.

Paper Details

Date Published: 26 January 2016
PDF: 6 pages
J. Biomed. Opt. 21(1) 016011 doi: 10.1117/1.JBO.21.1.016011
Published in: Journal of Biomedical Optics Volume 21, Issue 1
Show Author Affiliations
Chukwuemeka Okoro, Univ. of Illinois at Urbana-Champaign (United States)
Kimani C. Toussaint, Univ. of Illinois at Urbana-Champaign (United States)


© SPIE. Terms of Use
Back to Top