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Journal of Biomedical Optics

Nonspectroscopic imaging for quantitative chlorophyll sensing
Author(s): Taehoon Kim; Jeong-Im Kim; Michelle A. Visbal Onufrak; Clint Chapple; Young L. Kim
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Paper Abstract

Nondestructive imaging of physiological changes in plants has been intensively used as an invaluable tool for visualizing heterogeneous responses to various types of abiotic and biotic stress. However, conventional approaches often have intrinsic limitations for quantitative analyses, requiring bulky and expensive optical instruments for capturing full spectral information. We report a spectrometerless (or spectrometer-free) reflectance imaging method that allows for nondestructive and quantitative chlorophyll imaging in individual leaves <italic<in situ</italic< in a handheld device format. The combination of a handheld-type imaging system and a hyperspectral reconstruction algorithm from an RGB camera offers simple instrumentation and operation while avoiding the use of an imaging spectrograph or tunable color filter. This platform could potentially be integrated into a compact, inexpensive, and portable system, while being of great value in high-throughput phenotyping facilities and laboratory settings.

Paper Details

Date Published: 20 January 2016
PDF: 6 pages
J. Biomed. Opt. 21(1) 016008 doi: 10.1117/1.JBO.21.1.016008
Published in: Journal of Biomedical Optics Volume 21, Issue 1
Show Author Affiliations
Taehoon Kim, Purdue Univ. (United States)
Jeong-Im Kim, Purdue Univ. (United States)
Michelle A. Visbal Onufrak, Purdue Univ. (United States)
Clint Chapple, Purdue Univ. (United States)
Young L. Kim, Purdue Univ. (United States)
Kyung Hee Univ. (Korea, Republic of)


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