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Journal of Biomedical Optics

Spatially resolved, diffuse reflectance imaging for subsurface pattern visualization toward development of a lensless imaging platform: phantom experiments
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Paper Abstract

A portable, spatially resolved, diffuse reflectance lensless imaging technique based on the charge-coupled device or complementary metal-oxide semiconductor sensor directly coupled to the fiber optic bundle is proposed for visualization of subsurface structures such as superficial microvasculature in the epithelium. We discuss an experimental method for emulating a lensless imaging setup via raster scanning a single fiber-optic cable over a microfluidic phantom containing periodic hemoglobin absorption contrast. To evaluate the ability of the technique to recover information about the subsurface linear structures, scattering layers formed of the Sylgard® 184 Silicone Elastomer and titanium dioxide were placed atop the microfluidic phantom. Thickness of the layers ranged from 0.2 to 0.7 mm, and the values of the reduced scattering coefficient (μs) were between 0.85 and 4.25  mm1. The results demonstrate that fiber-optic, lensless platform can be used for two-dimensional imaging of absorbing inclusions in diffuse reflectance mode. In these experiments, it was shown that diffuse reflectance imaging can provide sufficient spatial sampling of the phantom for differentiation of 30  μm structural features of the embedded absorbing pattern inside the scattering media.

Paper Details

Date Published: 14 January 2016
PDF: 8 pages
J. Biomed. Opt. 21(1) 015004 doi: 10.1117/1.JBO.21.1.015004
Published in: Journal of Biomedical Optics Volume 21, Issue 1
Show Author Affiliations
Irina Schelkanova, Ryerson Univ. (Canada)
Aditya Pandya, Ryerson Univ. (Canada)
Guennadi Saiko, Ryerson Univ. (Canada)
Lidia Nacy, Ryerson Univ. (Canada)
Hannan Babar, Ryerson Univ. (Canada)
Duoaud F. Shah, Univ. Health Network (Canada)
Lothar Lilge, Univ. Health Network (Canada)
Univ. of Toronto (Canada)
Alexandre Douplik, The Keenan Research Ctr. (Canada)
Friedrich-Alexander-Univ. Erlangen-Nürnberg (Germany)

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