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Journal of Biomedical Optics • Open Access

Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera
Author(s): Carlos Cruz Perez; Antonella Lauri; Panagiotis Symvoulidis; Michele Cappetta; Arne Erdmann; Gil G. Westmeyer

Paper Abstract

Reconstructing a three-dimensional scene from multiple simultaneously acquired perspectives (the light field) is an elegant scanless imaging concept that can exceed the temporal resolution of currently available scanning-based imaging methods for capturing fast cellular processes. We tested the performance of commercially available light field cameras on a fluorescent microscopy setup for monitoring calcium activity in the brain of awake and behaving reporter zebrafish larvae. The plenoptic imaging system could volumetrically resolve diverse neuronal response profiles throughout the zebrafish brain upon stimulation with an aversive odorant. Behavioral responses of the reporter fish could be captured simultaneously together with depth-resolved neuronal activity. Overall, our assessment showed that with some optimizations for fluorescence microscopy applications, commercial light field cameras have the potential of becoming an attractive alternative to custom-built systems to accelerate molecular imaging research on cellular dynamics.

Paper Details

Date Published: 10 September 2015
PDF: 5 pages
J. Biomed. Opt. 20(9) 096009 doi: 10.1117/1.JBO.20.9.096009
Published in: Journal of Biomedical Optics Volume 20, Issue 9
Show Author Affiliations
Carlos Cruz Perez, Helmholtz Zentrum München GmbH (Germany)
Antonella Lauri, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)
Panagiotis Symvoulidis, Helmholtz Zentrum München GmbH (Germany)
Michele Cappetta, Helmholtz Zentrum München GmbH (Germany)
Technische Universität München (Germany)
Arne Erdmann, Raytrix GmbH (Germany)
Gil G. Westmeyer, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)

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