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Journal of Biomedical Optics

Development of photostabilized asymmetrical cyanine dyes for in vivo photoacoustic imaging of tumors
Author(s): Satoru Onoe; Takashi Temma; Kengo Kanazaki; Masahiro Ono; Hideo Saji
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Paper Abstract

Photoacoustic imaging (PAI) contributes to tumor diagnosis through the use of PAI probes that effectively accumulate in tumors. Previously, we developed a symmetrical cyanine dye, IC7-1-Bu, which showed high potential as a PAI probe because of its high tumor targeting ability and sufficient in vivo PA signal. However, IC7-1-Bu lacks photostability for multiple laser irradiations, so we developed stabilized PAI probes using IC7-1-Bu as a lead compound. We focused on the effect of singlet oxygen (1O2) generated by excited PAI probes on probe degeneration. We introduced a triplet-state quencher (TSQ) moiety into IC7-1-Bu to quench 1O2 generation and designed three IC-n-T derivatives with different linker lengths (n indicates linker length). The IC-n-T derivatives emitted in vitro PA signals that were comparable to IC7-1-Bu and significantly reduced 1O2 generation while showing improved photostability against multiple irradiations. Of the three derivatives evaluated, IC-5-T accumulated in tumors effectively to allow clear PAI of tumors in vivo. Furthermore, the photostability of IC-5-T was 1.5-fold higher than that of IC7-1-Bu in in vivo sequential PAI. These results suggest that IC-5-T is a potential PAI probe for in vivo sequential tumor imaging.

Paper Details

Date Published: 10 September 2015
PDF: 9 pages
J. Biomed. Opt. 20(9) 096006 doi: 10.1117/1.JBO.20.9.096006
Published in: Journal of Biomedical Optics Volume 20, Issue 9
Show Author Affiliations
Satoru Onoe, Kyoto Univ. (Japan)
Takashi Temma, Kyoto Univ. (Japan)
National Cerebral and Cardiovascular Ctr. (Japan)
Kengo Kanazaki, Canon Inc. (Japan)
Kyoto Univ. (Japan)
Masahiro Ono, Kyoto Univ. (Japan)
Hideo Saji, Kyoto Univ. (Japan)

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