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Journal of Biomedical Optics • Open Access

Estimation of multiexponential fluorescence decay parameters using compressive sensing
Author(s): Sejung Yang; Joohyun Lee; Youmin Lee; Minyung Lee; Byung-Uk Lee

Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments.

Paper Details

Date Published: 3 September 2015
PDF: 9 pages
J. Biomed. Opt. 20(9) 096003 doi: 10.1117/1.JBO.20.9.096003
Published in: Journal of Biomedical Optics Volume 20, Issue 9
Show Author Affiliations
Sejung Yang, Ewha Womans Univ. Medical Ctr. (Republic of Korea)
Joohyun Lee, Ewha Womans Univ. (Republic of Korea)
Youmin Lee, Ewha Womans Univ. (Republic of Korea)
Minyung Lee, Ewha Womans Univ. (Korea, Republic of)
Byung-Uk Lee, Ewha Womans Univ. (Republic of Korea)


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