Share Email Print
cover

Journal of Biomedical Optics

Optical properties of mouse brain tissue after optical clearing with FocusClear™
Author(s): Austin J. Moy; Bernard V. Capulong; Rolf B. Saager; Matthew P. Wiersma; Patrick C. Lo; Anthony J. Durkin; Bernard Choi
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Fluorescence microscopy is commonly used to investigate disease progression in biological tissues. Biological tissues, however, are strongly scattering in the visible wavelengths, limiting the application of fluorescence microscopy to superficial (<200  μm) regions. Optical clearing, which involves incubation of the tissue in a chemical bath, reduces the optical scattering in tissue, resulting in increased tissue transparency and optical imaging depth. The goal of this study was to determine the time- and wavelength-resolved dynamics of the optical scattering properties of rodent brain after optical clearing with FocusClear™. Light transmittance and reflectance of 1-mm mouse brain sections were measured using an integrating sphere before and after optical clearing and the inverse adding doubling algorithm used to determine tissue optical scattering. The degree of optical clearing was quantified by calculating the optical clearing potential (OCP), and the effects of differing OCP were demonstrated using the optical histology method, which combines tissue optical clearing with optical imaging to visualize the microvasculature. We observed increased tissue transparency with longer optical clearing time and an analogous increase in OCP. Furthermore, OCP did not vary substantially between 400 and 1000 nm for increasing optical clearing durations, suggesting that optical histology can improve ex vivo visualization of several fluorescent probes.

Paper Details

Date Published: 21 September 2015
PDF: 6 pages
J. Biomed. Opt. 20(9) 095010 doi: 10.1117/1.JBO.20.9.095010
Published in: Journal of Biomedical Optics Volume 20, Issue 9
Show Author Affiliations
Austin J. Moy, Univ. of California, Irvine (United States)
Bernard V. Capulong, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Rolf B. Saager, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Matthew P. Wiersma, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Patrick C. Lo, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Anthony J. Durkin, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Bernard Choi, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Edwards Lifesciences Ctr. for Advanced Cardiovascular Technology (United States)


© SPIE. Terms of Use
Back to Top