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Journal of Biomedical Optics • Open Access

Safety of cornea and iris in ocular surgery with 355-nm lasers
Author(s): Jenny Wang; Jae Lim Chung; Georg Schuele; Alexander Vankov; Roopa Dalal; Michael W. Wiltberger; Daniel Palanker

Paper Abstract

A recent study showed that 355-nm nanosecond lasers cut cornea with similar precision to infrared femtosecond lasers. However, use of ultraviolet wavelength requires precise assessment of ocular safety to determine the range of possible ophthalmic applications. In this study, the 355-nm nanosecond laser was evaluated for corneal and iris damage in rabbit, porcine, and human donor eyes as determined by minimum visible lesion (MVL) observation, live/dead staining of the endothelium, and apoptosis assay. Single-pulse damage to the iris was evaluated on porcine eyes using live/dead staining. In live rabbits, the cumulative median effective dose (ED50) for corneal damage was 231  J  /  cm2, as seen by lesion observation. Appearance of endothelial damage in live/dead staining or apoptosis occurred at higher radiant exposure of 287  J  /  cm2. On enucleated rabbit and porcine corneas, ED50 was 87 and 52  J  /  cm2, respectively, by MVL, and 241 and 160  J  /  cm2 for endothelial damage. In human eyes, ED50 for MVL was 110  J  /  cm2 and endothelial damage at 453  J  /  cm2. Single-pulse iris damage occurred at ED50 of 208  mJ  /  cm2. These values determine the energy permitted for surgical patterns and can guide development of ophthalmic laser systems. Lower damage threshold in corneas of enucleated eyes versus live rabbits is noted for future safety evaluation.

Paper Details

Date Published: 11 September 2015
PDF: 9 pages
J. Biomed. Opt. 20(9) 095005 doi: 10.1117/1.JBO.20.9.095005
Published in: Journal of Biomedical Optics Volume 20, Issue 9
Show Author Affiliations
Jenny Wang, Stanford Univ. (United States)
Jae Lim Chung, Stanford Univ. (United States)
Konyang Univ. (Republic of Korea)
Georg Schuele, Abbott Medical Optics (United States)
Alexander Vankov, Abbott Medical Optics (United States)
Roopa Dalal, Stanford Univ. (United States)
Michael W. Wiltberger, Abbott Medical Optics (United States)
Daniel Palanker, Stanford Univ. (United States)

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