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Journal of Biomedical Optics

Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant
Author(s): Shang Wang; Andrew L. Lopez; Kirill V. Larin; Paul A. Overbeek; Irina V. Larina
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Paper Abstract

Efficient phenotyping of developmental defects in model organisms is critical for understanding the genetic specification of normal development and congenital abnormalities in humans. We previously reported that optical coherence tomography (OCT) combined with live embryo culture is a valuable tool for mouse embryo imaging and four-dimensional (4-D) cardiodynamic analysis; however, its capability for analysis of mouse mutants with cardiac phenotypes has not been previously explored. Here, we report 4-D (three-dimensional+time) OCT imaging and analysis of the embryonic heart in a Wdr19 mouse mutant, revealing a heart looping defect. Quantitative analysis of cardiac looping revealed a statistically significant difference between mutant and control embryos. Our results indicate that live 4-D OCT imaging provides a powerful phenotyping approach to characterize embryonic cardiac function in mouse models.

Paper Details

Date Published: 21 September 2015
PDF: 4 pages
J. Biomed. Opt. 20(9) 090501 doi: 10.1117/1.JBO.20.9.090501
Published in: Journal of Biomedical Optics Volume 20, Issue 9
Show Author Affiliations
Shang Wang, Baylor College of Medicine (United States)
Andrew L. Lopez, Baylor College of Medicine (United States)
Kirill V. Larin, Baylor College of Medicine (United States)
Univ. of Houston (United States)
Samara State Aerospace Univ. (Russian Federation)
Paul A. Overbeek, Baylor College of Medicine (United States)
Irina V. Larina, Baylor College of Medicine (United States)

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