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Journal of Biomedical Optics

CT26 murine colon carcinoma expressing the red fluorescent protein KillerRed as a highly immunogenic tumor model
Author(s): Diana V. Yuzhakova; Marina V. Shirmanova; Ekaterina O. Serebrovskaya; Konstantin A. Lukyanov; Irina N. Druzhkova; Boris E. Shakhov; Sergey A. Lukyanov; Elena V. Zagaynova
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Paper Abstract

The development of tumor therapies based on the activation of antitumor immunity requires tumor models that are highly immunogenic. The immunologic response to fluorescent proteins, green fluorescent protein (GFP), or enhanced GFP (EGFP) was demonstrated in different cancer models. However, for live animal imaging, red and far-red fluorescent proteins are preferable, but their immunogenicity has not been studied. We assessed the immunogenicity of the red fluorescent protein, KillerRed (KR), in CT26 murine colon carcinoma. We showed a slower growth and a lower tumor incidence of KR-expressing tumors in comparison with nonexpressing ones. We found that KR-expressing lung metastases and rechallenged tumors were not formed in mice that had been surgically cured of KR-expressing primary tumors. The effect of low-dose cyclophosphamide (CY) treatment was also tested, as this is known to activate antitumor immune responses. The low-dose CY therapy of CT26-KR tumors resulted in inhibition of tumor growth and improved mouse survival. In summary, we have established a highly immunogenic tumor model that could be valuable for investigations of the mechanisms of antitumor immunity and the development of new therapeutic approaches.

Paper Details

Date Published: 17 August 2015
PDF: 7 pages
J. Biomed. Opt. 20(8) 088002 doi: 10.1117/1.JBO.20.8.088002
Published in: Journal of Biomedical Optics Volume 20, Issue 8
Show Author Affiliations
Diana V. Yuzhakova, Nizhny Novgorod State Medical Academy (Russia)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russia)
Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russia)
Ekaterina O. Serebrovskaya, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russia)
Konstantin A. Lukyanov, Nizhny Novgorod State Medical Academy (Russia)
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS (Russia)
Irina N. Druzhkova, Nizhny Novgorod State Medical Academy (Russia)
Boris E. Shakhov, Nizhny Novgorod State Medical Academy (Russia)
Sergey A. Lukyanov, Nizhny Novgorod State Medical Academy (Russia)
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS (Russia)
Pirogov Russian National Research Medical University (Russia)
Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)


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