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Journal of Biomedical Optics • Open Access

Quantitative phase imaging with molecular sensitivity using photoacoustic microscopy with a miniature ring transducer
Author(s): Adi Sheinfeld; Will J. Eldridge; Adam Wax

Paper Abstract

We present a dual-modality system for both structural and molecular cell imaging based on coregistered quantitative phase imaging (QPI) and photoacoustic microscopy (PAM). The QPI system was based on off-axis holography, whereas the PAM system comprised a sinusoidally modulated optical source for excitation and a narrow-band low profile and low-cost ring ultrasonic transducer for detection. This approach facilitated a simple confocal alignment of the excitation beams of both modalities and the ultrasonic detector. This system was demonstrated by imaging endogenous molecules in red blood cells (RBCs) as well as by imaging exogenous molecular labels on cancer cells using gold nanoparticles (GNPs) functionalized to target epidermal growth factor receptor. QPI provided high resolution imaging of the cellular structures while PAM provided molecular contrast. This dual-modality microscopy method can potentially be implemented as a compact and low cost cellular diagnostic assay.

Paper Details

Date Published: 11 August 2015
PDF: 6 pages
J. Biomed. Opt. 20(8) 086002 doi: 10.1117/1.JBO.20.8.086002
Published in: Journal of Biomedical Optics Volume 20, Issue 8
Show Author Affiliations
Adi Sheinfeld, Duke Univ. (United States)
Will J. Eldridge, Duke Univ. (United States)
Adam Wax, Duke Univ. (United States)

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