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Journal of Biomedical Optics

Probing intrinsic anisotropies of fluorescence: Mueller matrix approach
Author(s): Sudipta Saha; Nirmalya Ghosh; Jalpa Soni; Shubham Chandel; Uday Kumar
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Paper Abstract

We demonstrate that information on “intrinsic” anisotropies of fluorescence originating from preferential orientation/organization of fluorophore molecules can be probed using a Mueller matrix of fluorescence. For this purpose, we have developed a simplified model to decouple and separately quantify the depolarization property and the intrinsic anisotropy properties of fluorescence from the experimentally measured fluorescence Mueller matrix. Unlike the traditionally defined fluorescence anisotropy parameter, the Mueller matrix-derived fluorescence polarization metrics, namely, fluorescence diattenuation and polarizance parameters, exclusively deal with the intrinsic anisotropies of fluorescence. The utility of these newly derived fluorescence polarimetry parameters is demonstrated on model systems exhibiting multiple polarimetry effects, and an interesting example is illustrated on biomedically important fluorophores, collagen.

Paper Details

Date Published: 21 August 2015
PDF: 7 pages
J. Biomed. Opt. 20(8) 085005 doi: 10.1117/1.JBO.20.8.085005
Published in: Journal of Biomedical Optics Volume 20, Issue 8
Show Author Affiliations
Sudipta Saha, Indian Institute of Science Education and Research Kolkata (India)
Nirmalya Ghosh, Indian Institute of Science Education and Research Kolkata (India)
Jalpa Soni, Indian Institute of Science Education and Research Kolkata (India)
Shubham Chandel, Indian Institute of Science Education Research Kolkata (India)
Uday Kumar, Indian Institute of Science Education Research Kolkata (India)


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