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Journal of Biomedical Optics • Open Access

Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy
Author(s): Surekha Barkur; Aseefhali Bankapur; Madhura Pradhan; Santhosh Chidangil; Deepak Mathur; Uma Ladiwala

Paper Abstract

Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of OPO asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

Paper Details

Date Published: 5 August 2015
PDF: 9 pages
J. Biomed. Opt. 20(8) 085001 doi: 10.1117/1.JBO.20.8.085001
Published in: Journal of Biomedical Optics Volume 20, Issue 8
Show Author Affiliations
Surekha Barkur, Manipal Univ. (India)
Aseefhali Bankapur, Manipal Univ. (India)
Madhura Pradhan, Univ. of Mumbai (India)
Santhosh Chidangil, Manipal Univ. (India)
Deepak Mathur, Tata Institute of Fundamental Research (India)
Uma Ladiwala, Univ. of Mumbai (India)


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