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Journal of Biomedical Optics • Open Access

Raman spectroscopy for label-free identification of calciphylaxis
Author(s): William R. Lloyd; Shailesh Agarwal; Sagar U. Nigwekar; Shawn Fagan; Jeremy Goverman; Karen Esmonde-White; Shawn Loder; Bjorn R. Olsen; Dolrudee Jumlongras; Michael D. Morris; Benjamin Levi

Paper Abstract

Calciphylaxis is a painful, debilitating, and premorbid condition, which presents as calcified vasculature and soft tissues. Traditional diagnosis of calciphylaxis lesions requires an invasive biopsy, which is destructive, time consuming, and often leads to exacerbation of the condition and infection. Furthermore, it is difficult to find small calcifications within a large wound bed. To address this need, a noninvasive diagnostic tool may help clinicians identify ectopic calcified mineral and determine the disease margin. We propose Raman spectroscopy as a rapid, point-of-care, noninvasive, and label-free technology to detect calciphylaxis mineral. Debrided calciphylactic tissue was collected from six patients and assessed by microcomputed tomography (micro-CT). Micro-CT confirmed extensive deposits in three specimens, which were subsequently examined with Raman spectroscopy. Raman spectra confirmed that deposits were consistent with carbonated apatite, consistent with the literature. Raman spectroscopy shows potential as a noninvasive technique to detect calciphylaxis in a clinical environment.

Paper Details

Date Published: 11 August 2015
PDF: 3 pages
J. Biomed. Opt. 20(8) 080501 doi: 10.1117/1.JBO.20.8.080501
Published in: Journal of Biomedical Optics Volume 20, Issue 8
Show Author Affiliations
William R. Lloyd, Univ. of Michigan (United States)
Shailesh Agarwal, Univ. of Michigan Medical School (United States)
Sagar U. Nigwekar, Massachusetts General Hospital (United States)
Shawn Fagan, Massachusetts General Hospital (United States)
Jeremy Goverman, Massachusetts General Hospital (United States)
Karen Esmonde-White, Univ. of Michigan Medical School (United States)
Shawn Loder, Univ. of Michigan Medical School (United States)
Bjorn R. Olsen, Harvard School of Dental Medicine (United States)
Dolrudee Jumlongras, Harvard School of Dental Medicine (United States)
Michael D. Morris, Univ. of Michigan (United States)
Benjamin Levi, Univ. of Michigan Medical School (United States)

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