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Journal of Biomedical Optics

Photobiomodulation with low-level diode laser promotes osteoblast migration in an <italic<in vitro</italic< micro wound model
Author(s): Matilde Tschon; Serena Incerti-Parenti; Simona Cepollaro; Luigi Checchi; Milena Fini
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Paper Abstract

Laser photobiomodulation can improve bone healing, but well-defined treatment parameters are lacking. Saos-2 human osteoblast-like cells were subjected to an in vitro scratch-wound healing assay and irradiated by a 915-nm gallium-aluminum-arsenide diode laser for 0, 48, 96, and 144 s using doses of, respectively, 0, 5, 10, and 15  J/cm2. Wound area was measured after 4, 24, 48, and 72 h. Cell viability, DNA content, gene expression, and release of bone-related proteins were evaluated after 24, 48, and 72 h. Laser significantly improved wound healing compared with nonirradiated controls. Cells treated with laser doses of 5 and 10  J/cm2 reached wound closure after 72 h, followed by 15  J/cm2 after 96 h. With the cell proliferation inhibitor Mitomycin C, the doses of 10 and 15  J/cm2 maintained an improved wound healing compared with controls. Laser increased collagen type 1 gene expression with higher doses inducing a longer-lasting effect, whereas transforming growth factor-beta 1 showed comparable or decreased levels in irradiated versus nonirradiated groups, with no effect on protein release. This study demonstrated that laser photobiomodulation at 915 nm promoted wound healing mainly through stimulation of cell migration and collagen deposition by osteoblasts.

Paper Details

Date Published: 2 July 2015
PDF: 9 pages
J. Biomed. Opt. 20(7) 078002 doi: 10.1117/1.JBO.20.7.078002
Published in: Journal of Biomedical Optics Volume 20, Issue 7
Show Author Affiliations
Matilde Tschon, Rizzoli Orthopaedic Institute (Italy)
Serena Incerti-Parenti, Univ. degli Studi di Bologna (Italy)
Simona Cepollaro, Rizzoli Orthopaedic Institute (Italy)
Univ of Bologna (Italy)
Luigi Checchi, Univ. degli Studi di Bologna (Italy)
Milena Fini, Rizzoli Orthopaedic Institute (Italy)


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