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Journal of Biomedical Optics

Confocal Raman microscopy to monitor extracellular matrix during dental pulp stem cells differentiation
Author(s): Hamideh Salehi; Pierre-Yves Collart-Dutilleul; Csilla Gergely; Frédéric J. G. Cuisinier
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Paper Abstract

Regenerative medicine brings promising applications for mesenchymal stem cells, such as dental pulp stem cells (DPSCs). Confocal Raman microscopy, a noninvasive technique, is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800 to 3000  cm−1 region (C-H stretching) and the 960  cm−1 peak (ν1PO43) were collected (to image cells and phosphate, respectively), and the ratio of two peaks 1660 over 1690  cm−1 (amide I bands) to measure the collagen cross-linking has been calculated. Raman spectra of DPSCs after 21 days differentiation reveal several phosphate peaks: ν1 (first stretching mode) at 960  cm−1, ν2 at 430  cm−1, and ν4 at 585  cm−1 and collagen cross-linking can also be calculated. Confocal Raman microscopy enables monitoring osteogenic differentiation in vitro and can be a credible tool for clinical stem cell based research.

Paper Details

Date Published: 27 July 2015
PDF: 6 pages
J. Biomed. Opt. 20(7) 076013 doi: 10.1117/1.JBO.20.7.076013
Published in: Journal of Biomedical Optics Volume 20, Issue 7
Show Author Affiliations
Hamideh Salehi, Univ. Montpellier (France)
Pierre-Yves Collart-Dutilleul, Univ. Montpellier 1 (France)
Csilla Gergely, Univ. Montpellier 2 (France)
Frédéric J. G. Cuisinier, Univ. Montpellier 1 (France)


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