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Journal of Biomedical Optics

Evaluation of light scattering and absorption properties of in vivo rat liver using a single-reflectance fiber probe during preischemia, ischemia–reperfusion, and postmortem
Author(s): Sharmin Akter; Satoshi Maejima; Satoko Kawauchi; Shunichi Sato; Akinari Hinoki; Suefumi Aosasa; Junji Yamamoto; Izumi Nishidate
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Paper Abstract

Diffuse reflectance spectroscopy (DRS) has been extensively used for characterization of biological tissues as a noninvasive optical technique to evaluate the optical properties of tissue. We investigated a method for evaluating the reduced scattering coefficient μs, the absorption coefficient μa, the tissue oxygen saturation StO2, and the reduction of heme aa3 in cytochrome c oxidase CcO of in vivo liver tissue using a single-reflectance fiber probe with two source-collector geometries. We performed in vivo recordings of diffuse reflectance spectra for exposed rat liver during the ischemia–reperfusion induced by the hepatic portal (hepatic artery, portal vein, and bile duct) occlusion. The time courses of μa at 500, 530, 570, and 584 nm indicated the hemodynamic change in liver tissue as well as StO2. Significant increase in μa(605)/μa(620) during ischemia and after euthanasia induced by nitrogen breathing was observed, which indicates the reduction of heme aa3, representing a sign of mitochondrial energy failure. The time courses of μs′ at 500, 530, 570, and 584 nm were well correlated with those of μa, which also reflect the scattering by red blood cells. On the other hand, at 700 and 800 nm, a temporary increase in μs and an irreversible decrease in μs were observed during ischemia–reperfusion and after euthanasia induced by nitrogen breathing, respectively. The change in μs in the near-infrared wavelength region during ischemia is indicative of the morphological changes in the cellular and subcellular structures induced by the ischemia, whereas that after euthanasia implies the hepatocyte vacuolation. The results of the present study indicate the potential application of the current DRS system for evaluating the pathophysiological conditions of in vivo liver tissue.

Paper Details

Date Published: 27 July 2015
PDF: 12 pages
J. Biomed. Opt. 20(7) 076010 doi: 10.1117/1.JBO.20.7.076010
Published in: Journal of Biomedical Optics Volume 20, Issue 7
Show Author Affiliations
Sharmin Akter, Tokyo Univ. of Agriculture and Technology (Japan)
Satoshi Maejima, National Defense Medical College (Japan)
Satoko Kawauchi, National Defense Medical College (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Akinari Hinoki, National Defense Medical College (Japan)
Suefumi Aosasa, National Defense Medical College (Japan)
Junji Yamamoto, National Defense Medical College (Japan)
Izumi Nishidate, Tokyo Univ. of Agriculture and Technology (Japan)

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