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Journal of Biomedical Optics

On involvement of transcription factors nuclear factor kappa-light-chain-enhancer of activated B cells, activator protein-1 and signal transducer and activator of transcription-3 in photodynamic therapy-induced death of crayfish neurons and satellite glial cells
Author(s): Elena Berezhnaya; Marya A. Neginskaya; Vera D. Kovaleva; Svetlana Sharifulina; Irina Ischenko; Maxim A Komandirov; Mikhail Rudkovskii; Anatoly B. Uzdensky
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Paper Abstract

Photodynamic therapy (PDT) is currently used in the treatment of brain tumors. However, not only malignant cells but also neighboring normal neurons and glial cells are damaged during PDT. In order to study the potential role of transcription factors—nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), activator protein (AP-1), and signal transducer and activator of transcription-3 (STAT-3)—in photodynamic injury of normal neurons and glia, we photosensitized the isolated crayfish mechanoreceptor consisting of a single sensory neuron enveloped by glial cells. Application of different inhibitors and activators showed that transcription factors NF-κB (inhibitors caffeic acid phenethyl ester and parthenolide, activator betulinic acid), AP-1 (inhibitor SR11302), and STAT-3 (inhibitors stattic and cucurbitacine) influenced PDT-induced death and survival of neurons and glial cells in different ways. These experiments indicated involvement of NF-κB in PDT-induced necrosis of neurons and apoptosis of glial cells. However, in glial cells, it played the antinecrotic role. AP-1 was not involved in PDT-induced necrosis of neurons and glia, but mediated glial apoptosis. STAT-3 was involved in PDT-induced apoptosis of glial cells and necrosis of neurons and glia. Therefore, signaling pathways that regulate cell death and survival in neurons and glial cells are different. Using various inhibitors or activators of transcription factors, one can differently influence the sensitivity and resistance of neurons and glial cells to PDT.

Paper Details

Date Published: 9 July 2015
PDF: 9 pages
J. Biomed. Opt. 20(7) 075004 doi: 10.1117/1.JBO.20.7.075004
Published in: Journal of Biomedical Optics Volume 20, Issue 7
Show Author Affiliations
Elena Berezhnaya, Southern Federal Univ. (Russia)
Marya A. Neginskaya, Southern Federal Univ. (Russia)
Vera D. Kovaleva, Southern Federal Univ. (Russia)
Svetlana Sharifulina, Southern Federal Univ. (Russia)
Irina Ischenko, Southern Federal Univ. (Russia)
Maxim A Komandirov, Southern Federal Univ. (Russia)
Mikhail Rudkovskii, Southern Federal Univ. (Russia)
Anatoly B. Uzdensky, Southern Federal Univ. (Russia)

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