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Journal of Biomedical Optics

Using infrared and Raman microspectroscopies to compare ex vivo involved psoriatic skin with normal human skin
Author(s): Marie Leroy; Thierry Lefèvre; Roxane Pouliot; Michèle Auger; Gaétan Laroche
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Paper Abstract

Psoriasis is a chronic dermatosis that affects around 3% of the world’s population. The etiology of this autoimmune pathology is not completely understood. The barrier function of psoriatic skin is known to be strongly altered, but the structural modifications at the origin of this dysfunction are not clear. To develop strategies to reduce symptoms of psoriasis or adequate substitutes for modeling, a deep understanding of the organization of psoriatic skin at a molecular level is required. Infrared and Raman microspectroscopies have been used to obtain direct molecular-level information on psoriatic and healthy human skin biopsies. From the intensities and positions of specific vibrational bands, the lipid and protein distribution and the lipid order have been mapped in the different layers of the skin. Results showed a similar distribution of lipids and collagen for normal and psoriatic human skin. However, psoriatic skin is characterized by heterogeneity in lipid/protein composition at the micrometer scale, a reduction in the definition of skin layer boundaries and a decrease in lipid chain order in the stratum corneum as compared to normal skin. A global decrease of the structural organization is exhibited in psoriatic skin that is compatible with an alteration of its barrier properties.

Paper Details

Date Published: 17 June 2015
PDF: 10 pages
J. Biomed. Opt. 20(6) 067004 doi: 10.1117/1.JBO.20.6.067004
Published in: Journal of Biomedical Optics Volume 20, Issue 6
Show Author Affiliations
Marie Leroy, Univ. Laval (Canada)
Ctr. Hospitalier Univ. de Québec (Canada)
Thierry Lefèvre, Univ. Laval (Canada)
Roxane Pouliot, Univ. Laval (Canada)
Michèle Auger, Univ. Laval (Canada)
Gaétan Laroche, Univ. Laval (Canada)
Ctr. Hospitalier Univ. de Québec (Canada)

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