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Journal of Biomedical Optics

Incubator proof miniaturized Holomonitor to <italic<in situ</italic< monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections
Author(s): Agnes Dobos; Beatrix Peter; Judit Nador; Krisztina Juhasz; Inna Székács; Daniel Patko; Robert Horvath; Laszlo Korosi
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Paper Abstract

The <italic<in situ</italic< observation of cell movements and morphological parameters over longer periods of time under physiological conditions is critical in basic cell research and biomedical applications. The quantitative phase-contrast microscope applied in this study has a remarkably small size, therefore it can be placed directly into a humidified incubator. Here, we report on the successful application of this M4 Holomonitor to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications. A special mechanical stage was developed to position the sample into that range of the optical arrangement where digital autofocusing works with high reproducibility and precision. By in-depth analyzing the obtained single cell morphological parameters, we show that the limited vertical resolution of the optical setup results in underestimated single cell contact area and volume and overestimated single cell averaged thickness. We propose a simple model to correct the recorded data to obtain more precise single cell parameters. We compare the results with the kinetic data recorded by a surface sensitive optical biosensor, optical waveguide lightmode spectroscopy.

Paper Details

Date Published: 9 June 2015
PDF: 8 pages
J. Biomed. Opt. 20(6) 067002 doi: 10.1117/1.JBO.20.6.067002
Published in: Journal of Biomedical Optics Volume 20, Issue 6
Show Author Affiliations
Agnes Dobos, Institute for Technical Physics and Materials Science (Hungary)
Beatrix Peter, Univ. of Pannonia (Hungary)
Institute for Technical Physics and Materials Science (Hungary)
Judit Nador, Univ. of Pannonia (Hungary)
Institute for Technical Physics and Materials Science (Hungary)
Krisztina Juhasz, Univ. of Pannonia (Hungary)
Institute for Technical Physics and Materials Science (Hungary)
Inna Székács, Institute for Technical Physics and Materials Science (Hungary)
Daniel Patko, Univ. of Pannonia (Hungary)
Institute for Technical Physics and Materials Science (Hungary)
Robert Horvath, Univ. of Pannonia (Hungary)
Institute for Technical Physics and Materials Science (Hungary)
Laszlo Korosi, Enviroinvest Ltd. (Hungary)


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