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Journal of Biomedical Optics

Determination of biodistribution of ultrasmall, near-infrared emitting gold nanoparticles by photoacoustic and fluorescence imaging
Author(s): Wilson Poon; Andrew Heinmiller; Xuan Zhang; Jay L. Nadeau
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Paper Abstract

This study compares fluorescence and photoacoustic (PA) imaging of ex vivo tumors and organs from tumor-bearing mice injected intravenously with ultrasmall (<3  nm) tiopronin-capped Au nanoparticles and compares the data with inductively coupled plasma mass spectrometry (ICP-MS). Good agreement is seen in particle distributions and concentrations at the organ level. The spatial resolution from the imaging techniques allows for localization of the particles within organ structures. Although the particles do not have a plasmon peak, their absorbance in the near-infrared (NIR) is sufficient for PA excitation. PA imaging shows an increase of signal as particle concentrations increase, with changes in spectrum if particles aggregate. Fluorescence imaging using the particles’ native NIR emission shows agreement in general intensity in each organ, though quenching of emission can be seen at very high concentrations. Both of these imaging techniques are noninvasive and labor-saving alternatives to organ digestion and ICP-MS and may provide insight into cellular distribution of particles. The simple construct avoids the use of toxic semiconductor materials or dyes, relying upon the gold itself for both the fluorescence and PA signal. This provides a useful alternative to more complex approaches to multimodal imaging and one that is readily translatable to the clinic.

Paper Details

Date Published: 23 June 2015
PDF: 8 pages
J. Biomed. Opt. 20(6) 066007 doi: 10.1117/1.JBO.20.6.066007
Published in: Journal of Biomedical Optics Volume 20, Issue 6
Show Author Affiliations
Wilson Poon, McGill Univ. (Canada)
Univ. of Toronto (Canada)
Andrew Heinmiller, FUJIFILM VisualSonics, Inc. (Canada)
Xuan Zhang, McGill Univ. (Canada)
Jay L. Nadeau, McGill University (Canada)

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